factor 1 sdf1 antibody  (Abcam)

Journal: Cancers

Article Title: Epithelial and Stromal Characteristics of Primary Tumors Predict the Bone Metastatic Subtype of Prostate Cancer and Patient Survival after Androgen-Deprivation Therapy

doi: 10.3390/cancers14215195

Figure Lengend Snippet: Sections from paired primary prostate tumors and bone metastases immune stained for ETS-related gene (ERG) ( a , h ), prostate specific antigen (PSA) ( b ), the marker for proliferation Ki67 ( c ), androgen receptor (AR) ( d ), smooth muscle actin (SMA) ( e ), stroma derived factor 1 (SDF1) ( f ), and platelet-derived growth factor receptor β (PDGFRB) ( g ), all seen at the same magnification (see scalebar in ( a )). ( a ) ERG positive tumor cells in a primary tumor and in the paired metastasis. ( b ) Moderate (left side) to high (right side with glands) PSA staining in a primary tumor. Moderate PSA staining and glandular differentiation was seen in the metastasis (classified as MetA). ( c ) Sections with relatively high Ki67 labeling in epithelium and stroma in a primary tumor and in the paired bone metastasis. ( d ) AR positive epithelial and stroma cells were seen in the primary tumor. In the metastasis (classified as MetA), a nest of AR positive tumor cells was growing in a bone marrow space and a few AR positive cells are seen on the bone surface. ( e ) Numerous SMA positive smooth muscle cells are seen in the primary tumor, whereas only few cells were SMA positive in the paired bone metastasis (here only in blood vessel walls). This metastasis, lacking bone structures, was classified as MetB. ( f ) SDF-1 positive cells were seen in the stroma in the primary tumor, mainly in blood vessel walls. Such cells were also seen in the bone metastasis stroma. ( g ) PDGFRB positive stroma cells, mainly in blood vessel walls and in fibroblasts, were seen in the primary tumor stroma and in the paired metastasis (classified as MetB). ( h ) ERG positive endothelial cell nuclei were seen in the primary tumor and in its bone metastasis. Note that several tumor cells are lying close to endothelial cells. In this case, the tumor cells were ERG negative. This metastasis was classified as Ki67 high/PSA low but with unknown MetA-C status. Blood vessels with ERG positive endothelial cells and walls positive for SMA, PDGFR-beta, and SDF-1 were a dominant component of the bone metastasis stroma.

Article Snippet: Stroma derived factor 1 (SDF1) (MAB350/clone 79018, R&D Systems, Minneapolis, MN, US) 1:50 overnight) was stained manually using Tris-EDTA pH9 as antigen retrieval and developed with DAB (Mach3Mouse HRP-Polymer Detection, M3M530, Biocare Medical, Pacheco, CA, US).

Techniques: Staining, Marker, Derivative Assay, Labeling

Journal: Cells

Article Title: Improved Method for Dental Pulp Stem Cell Preservation and Its Underlying Cell Biological Mechanism

doi: 10.3390/cells12172138

Figure Lengend Snippet: Immunohistochemical localization of SDF1 in dental pulp tissue cultured using the Improved CMDPT. ( a – d ) Immunohistochemical staining for SDF1 (( c , d ) are high magnification images of the red box area, respectively), ( e , f ) Negative control. ( a , c , e ) After 72 h of culture, ( b , d , f ). After 96 h of culture. SDF1 expression was observed in the center portion of the dental pulp tissue after 72 h of culture when active cell migration was observed. In contrast, no expression of SDF1 was observed at 96 h of culture, when almost all of the cells had migrated vertically toward the membrane. Arrowheads indicate the localization of SDF1. ( a – f ) Scale bars are 50 µm.

Article Snippet: After treatment with 10% normal goat serum at RT for 10 min, the sections were incubated with rabbit anti-Ki-67 antibody (Abcam, Cambridge, UK) (diluted 1:200 in PBS containing 1% bovine serum albumin) or rabbit anti-stromal cell-derived factor-1(SDF1) antibody (Abcam, Cambridge, UK) (diluted 1:1000 in PBS containing 1% bovine serum albumin) at 4 °C overnight.

Techniques: Immunohistochemistry, Cell Culture, Staining, Negative Control, Expressing, Migration, Membrane

Journal: Cells

Article Title: Improved Method for Dental Pulp Stem Cell Preservation and Its Underlying Cell Biological Mechanism

doi: 10.3390/cells12172138

Figure Lengend Snippet: Effect of SDF1 on the migration of DPSCs in vitro ( a ) Photo of the culture insert. ( b ) The number of migrating cells. ( c ) The absorbance of crystal violet at 570 nm. A significant cell-migration-promoting effect of SDF1 was observed in the culture of DPSCs.

Article Snippet: After treatment with 10% normal goat serum at RT for 10 min, the sections were incubated with rabbit anti-Ki-67 antibody (Abcam, Cambridge, UK) (diluted 1:200 in PBS containing 1% bovine serum albumin) or rabbit anti-stromal cell-derived factor-1(SDF1) antibody (Abcam, Cambridge, UK) (diluted 1:1000 in PBS containing 1% bovine serum albumin) at 4 °C overnight.

Techniques: Migration, In Vitro

Journal: Cells

Article Title: Improved Method for Dental Pulp Stem Cell Preservation and Its Underlying Cell Biological Mechanism

doi: 10.3390/cells12172138

Figure Lengend Snippet: Effect of SDF1-neutralizing antibody on the migration of DPSCs under Improved CMDPT. Histological images after 48 h ( a , d ), 72 h ( b , e ), 96 h ( c , f ) of cultures using the Improved CMDPT. ( a – f ) H&E staining. ( a – c ) Control. ( d – f ) SDF1-neutralizing antibody was added. After 48 h of culture, DPSCs were localized near the membrane in the culture without an SDF1-neutralizing antibody. In contrast, no cell migration was observed in the presence of SDF1-neutralizing antibody. After 72 h of culture, DPSCs migrated toward the membranes, whereas no migration was observed in the cultures containing SDF1-neutralizing antibody. After 96 h of culture, the cells in the control group, which had been localized in the vicinity of the membrane after 72 h of culture, were almost absent, presumably because of growth outside the dental pulp tissue. In contrast, in the SDF1-neutralizing-antibody-treated group, some DPSCs were localized near the upper and lower membranes. ( a – f ) Scale bars are 50 µm.

Article Snippet: After treatment with 10% normal goat serum at RT for 10 min, the sections were incubated with rabbit anti-Ki-67 antibody (Abcam, Cambridge, UK) (diluted 1:200 in PBS containing 1% bovine serum albumin) or rabbit anti-stromal cell-derived factor-1(SDF1) antibody (Abcam, Cambridge, UK) (diluted 1:1000 in PBS containing 1% bovine serum albumin) at 4 °C overnight.

Techniques: Migration, Staining, Membrane

Journal: Frontiers in Immunology

Article Title: Head and neck dermatitis is exacerbated by Malassezia furfur colonization, skin barrier disruption, and immune dysregulation

doi: 10.3389/fimmu.2023.1114321

Figure Lengend Snippet: Immunohistochemistry of HND and non-HND facial skin specimens. (A) Representative histological image at 100x magnification (left) and 200x magnification (right). Quantified staining intensity of (B) SDF-1α, (C) IL-1β, (D) TGF-β, (E) TNF-α, and (F) VEGF. (Unpaired two-tailed t-test, SDF-1α; p = 0.0014, IL-1β; p = 0.0374, TGF-β; p = 0.0058, TNF-α; p = 0.0035, VEGF; p = 0.0002) HND, head and neck dermatitis; SDF-1 α , stromal cell-derived factor-1-alpha; IL-1 β , Interleukin-1-beta; TGF- β , transforming growth factor-beta; TNF- α , tumor necrosis factor-alpha; VEGF, vascular endothelial growth factor. * : p<0.05; ** : p<0.01.

Article Snippet: Immunohistochemical staining was performed using paraffin-embedded sections with antibodies against factor VIII-related antigen (1:100, ab236284, Abcam), stromal cell-derived factor-1-alpha (SDF1-α) (1:100, ab25117, Abcam, Cambridge, United Kingdom), Interleukin-1-beta (IL-1-β) (1:100, ab2105, Abcam), tumor necrosis factor-alpha (TNF-α) (1:50, ab1793, Abcam), transforming growth factor-beta (TGF-β) (1:100, ab66043, Abcam), and vascular endothelial growth factor (VEGF) (1:200, ab1316, Abcam).

Techniques: Immunohistochemistry, Staining, Two Tailed Test, Derivative Assay

Journal: PLoS ONE

Article Title: FOXO4-Knockdown Suppresses Oxidative Stress-Induced Apoptosis of Early Pro-Angiogenic Cells and Augments Their Neovascularization Capacities in Ischemic Limbs

doi: 10.1371/journal.pone.0092626

Figure Lengend Snippet: (A) Representative fluorescence microscopic images of EPCs incorporated in endothelial cells of the ischemic limbs of athymic nude rats 60 h after intramuscular injection of EPCs, NT-EPCs, or FOXO4 KD -EPCs. Rat specific CD31-positive endothelial cells were stained red. Human specific PCNA-positive cells, which were defined as human-derived EPCs, were stained green. Scale bar: 50 μm. (B) Pooled data of the number of PCNA-positive EPCs incorporated in endothelial cells of the rat ischemic limbs 60 h after the injection of EPCs, NT-EPCs, or FOXO4 KD -EPCs (*: p<0.01; **: p<0.005; n = 5, each). (C) A representative western blotting photo of expressions of human specific VEGF, b-FGF, SDF-1, and IGF-1 in the rat ischemic limb 60 h after intramuscular injection of EPCs, NT-EPCs, or FOXO4 KD -EPCs (n = 4, each).

Article Snippet: The membrane was blocked with a buffer (Blocking One, Nacalai Tesque, Inc.) and incubated for 24 h at 4°C with primary antibodies as follows: a rabbit anti-human β-actin antibody, a rabbit anti-human FOXO3a antibody, a rabbit anti-human FOXO4 antibody, a rabbit anti-human Bim antibody, a rabbit anti-human cleaved caspase-3 antibody, a rabbit anti-human vascular endothelial growth factor (VEGF) antibody, a rabbit anti-human basic-fibroblast growth factor (b-FGF) antibody (above antibodies were purchased from Cell Signaling Technology), a rabbit anti-human stromal cell-derived factor-1 (SDF-1) antibody, and a rabbit anti-human insulin-like growth factor-1 (IGF-1) antibody (above antibodies were purchased from abcam).

Techniques: Fluorescence, Injection, Staining, Derivative Assay, Western Blot

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Combination of Ligusticum Chuanxiong and Radix Paeonia Promotes Angiogenesis in Ischemic Myocardium through Notch Signalling and Mobilization of Stem Cells

doi: 10.1155/2019/7912402

Figure Lengend Snippet: Expression of angiogenesis and stem cell mobilization proteins. (a) Protein levels of HIF-1 α and FGFR-1. (b) Protein levels of SDF-1, CXCR-4, and cardiotrophin1. (c) Protein levels of Notch1 and NICD. ∗ P <0.05, ∗∗ P <0.01, and ∗∗∗ P <0.001 vs. sham group; $ P <0.05, $$ P <0.01 vs. model group; && P <0.01 vs. CX-CS group. n=3.

Article Snippet: After incubation with primary antibodies against GAPDH (5174, CST, USA), hypoxia-inducible factor 1-alpha (HIF-1 α ) (36169, CST, USA), FGF Receptor 1 (FGFR-1) (9740, CST, USA), Notch1 (3608, CST, USA), cleaved Notch1 (also named NICD) (4147s, CST), stromal cell-derived factor-1 (SDF-1) (3740, CST, USA), C-X-C chemokine receptor 4 (CXCR-4) (60042-1-1g, Proteintech, USA), and cardiotrophin1 (YT0638, ImmunoWay, USA) at 4°C, the membranes were incubated with a secondary antibody (115-035-003, Jackson, USA) at room temperature for two hours.

Techniques: Expressing